HPLC is one of the several avatars of Chromatography technique that is being profusely utilised in the laboratories all over the world. Conventionally, this technique is used to separate molecules of comparable mass and run time. Comprising of a mobile medium, a static medium and a chromatogram, this technique operates on the principle of capillary action. The heavier the molecule, the slower it climbs up the static medium. In HPLC, the static medium is invariably a silicone porous layer. H...


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