HPLC is one of the several avatars of Chromatography technique that is being
profusely utilised in the laboratories all over the world. Conventionally, this
technique is used to separate molecules of comparable mass and run time.
Comprising of a mobile medium, a static medium and a chromatogram, this
technique operates on the principle of capillary action. The heavier the
molecule, the slower it climbs up the static medium. In HPLC, the static medium
is invariably a silicone porous layer. H...
Continue reading ...